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KMID : 1098420220300060430
Korean Journal of Medicinal Crop Science
2022 Volume.30 No. 6 p.430 ~ p.439
Development of a Chloroplast-based InDel Marker that Discriminates between Paeonia suffruticosa and P. lactiflora
Lee Mi-Sun

Jeong Hee-Jeong
Park So-Hyeon
Chung Hee
Jeong Jin-Tae
Kim Moon-Kyo
Gil Jin-Su
Lee Jae-Bok
Kim Se-Rim
Yun Ki-Hoon
Lee Yi
Abstract
Background: Paeonia suffruticosa Andrew and P. lactiflora Pallas are important medicinal plants that are difficult to differentiate due to their similar appearances. The development of molecular markers will aid in the discrimination of these two species.

Methods and Results: We found several chloroplast genomic loci that were polymorphic between P. suffruticosa and P. lactiflora. Among them, the insertion/deletion (InDel) marker PsPl-InDel-12 clearly discriminated 25 P. suffruticosa and P. lactiflora samples collected in the Republic of Korea. We were able to discriminate the two species in material of mixed status.

Conclusions: The marker developed in this study can be used to ensure P. suffruticosa and P. lacti?flora quality and increase consumer confidence when purchasing medicinal products made from these species.
KEYWORD
Paeonia lactiflora, Paeonia suffruticosa, Chloroplast, Genotyping, Insertion/deletion Marker
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