KMID : 1098420220300060430
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Korean Journal of Medicinal Crop Science 2022 Volume.30 No. 6 p.430 ~ p.439
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Development of a Chloroplast-based InDel Marker that Discriminates between Paeonia suffruticosa and P. lactiflora
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Lee Mi-Sun
Jeong Hee-Jeong Park So-Hyeon Chung Hee Jeong Jin-Tae Kim Moon-Kyo Gil Jin-Su Lee Jae-Bok Kim Se-Rim Yun Ki-Hoon Lee Yi
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Abstract
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Background: Paeonia suffruticosa Andrew and P. lactiflora Pallas are important medicinal plants that are difficult to differentiate due to their similar appearances. The development of molecular markers will aid in the discrimination of these two species.
Methods and Results: We found several chloroplast genomic loci that were polymorphic between P. suffruticosa and P. lactiflora. Among them, the insertion/deletion (InDel) marker PsPl-InDel-12 clearly discriminated 25 P. suffruticosa and P. lactiflora samples collected in the Republic of Korea. We were able to discriminate the two species in material of mixed status.
Conclusions: The marker developed in this study can be used to ensure P. suffruticosa and P. lacti?flora quality and increase consumer confidence when purchasing medicinal products made from these species.
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KEYWORD
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Paeonia lactiflora, Paeonia suffruticosa, Chloroplast, Genotyping, Insertion/deletion Marker
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